What is this?

2024.11.30 03:29 Ok_Mud_2820 What is this?

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2024.11.30 03:29 Low-Broccoli-157 Voided bet

Had Jaylen brown over 3.5 assists tonight. His fourth assist came as a missed three point shot, but was tipped in ( in the air) for a basket. Score keepers at the game, gave him an assist. Draft kings voided that leg of a parlay. wtf?
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2024.11.30 03:29 Anipiez What would you make uncanon?

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2024.11.30 03:29 lucidcxnt I’m new to this

I lost my big brother (21) on November 20th, he passed due to a drug overdose and an aneurysm after being sober for 6+ months. I’ve grieved people and loved ones in the past before but nobodies death has hit me quite this hard.. He was such a bright soul and he had such a warm and welcoming smile and aura to him. He was literally sunshine in person form. I feel so guilty for not seeing him a lot more before he died. I don’t know how to properly grieve/accept his death. I’m currently almost 35 weeks pregnant and have a 1 year old so I’ve been busy preparing for birth and keeping my 1 year old busy and preparing her to be a big sister. All I can see when I think of him is him laying in his ICU bed on life support. I think of him when my house gets too quiet or when I sit still for too long. I text him expecting an answer and of course never get one. Anticipating his texts back is what’s killing me. I just want to hear his voice one more time. I want to hold him and smell him one more time.
It’s like my brain can’t comprehend that he is not living anymore, I catch myself thinking that he’s just busy at work and he’ll text me later on. I’m sorry for rambling, I’m just so jumbled up right now and have no idea what to think.
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2024.11.30 03:29 Nitroso-etherealist Sigma-1 and Nicotinic relationship and downstream targets (continued)

PC12 Mutagenized cell variants reveal striking differences in the expression of Sodium channels and Nicotinic receptors when compared to wild-type PC12 cells. Even in the absence of nerve growth factor (NGF), the mutant cells express functional Na channels and Na channel mRNA at levels exceeding those in wild-type PC12 cells differentiated with NGF. In contrast, acetylcholine-induced currents were evident in only a small proportion of cells, presumably due to the altered pattern of expression of mRNAs encoding individual nAChR subunits. The altered ion channel expression in these variants provides an avenue for analyzing Na channel and nAChR channel function, as well as for identifying mechanisms governing their expression. https://link.springer.com/article/10.1007/BF00238418
Interactions in Dynamic reciprocity of sodium and potassium channel expression in a macromolecular complex involve Sigma-1 receptors. Such as Sigma-1 mediated regulation of ion currents interacting with various channels, like Kv1.2 channels, increasing their surface expression. Sigma-1 also inhibit Kv1.3, Kv1.4, and Kv1.5 channels. While regulating Kv2.1 channels, and inhibiting L-type voltage-gated calcium channels. Also inhibiting N-type Ca2+ channels currents. And inhibit voltage-gated sodium ion channels: Nav1.2/1.4 and Nav1.5. Sigma-1 regulate hERG channels while inhibiting acid-sensing ion channels (ASIC1a). https://www.sciencedirect.com/science/article/pii/S0149763421004796
NGF-dependent increase in phosphoinositide hydrolysis in PC12 cells is due to selective phosphorylation of PLC-gamma by serine and tyrosine protein kinases associated with the NGF receptor. https://www.sciencedirect.com/science/article/pii/S0021925818522997
Dose-Response Curve Stimulation by ATP gamma S of phospholipase C(PLC) was shifted to the right by the presence of UTP, indicating that both compounds act on the same receptors. Neuronal “nucleotide” receptor linked to phospholipase C and phospholipase D.
The lipase activity of PLCβ is not required for C3PO inhibition, and C3PO does not inhibit PLCβ. C3 Protein reduces neurite outgrowth and neuronal viability in vitro and restricts axon regeneration in vivo, and demonstrate a novel, non-traditional role for this inflammatory protein in the central nervous system.
PLCβ1 is strongly activated by Gαq. NGF added to PC12 cells remarkably increased PLCβ1 (i.e. 2.5–2.7-fold) in first 24 h. This increase peaks to 4-fold at 48 h (Fig. 1A) and decreases thereafter. Although Gαq also showed a large increase in expression with differentiation (1.6-fold), the onset of this increase was delayed 24 h relative to PLCβ
Alpha-7 Nicotinic Agonists or PAM both increased RhoA activity and inhibited neurite outgrowth. G-Protein Coupling is necessary for Alpha-7 Nicotinic mediated activated of RhoA. https://onlinelibrary.wiley.com/doi/epdf/10.1111/jnc.13660
Sigma-1 receptor agonist induces RhoA/ROCK activation. https://www.atherosclerosis-journal.com/article/S0021-9150(16)31056-5/abstract
Sigma-1 agonist significantly increased both β-catenin and ZO-1 levels. https://pmc.ncbi.nlm.nih.gov/articles/PMC7821090/
Wnt/β-catenin signaling plays an essential role in α7 nicotinic receptor-mediated neuroprotection. https://www.sciencedirect.com/science/article/abs/pii/S0006295217302848
Nicotinic ACh receptor activation decreases IL-1β secretion. stimulation of the evolutionarily ancient CHRNA7, CHRNA9, and/or CHRNA10 efficiently inhibit ATP-mediated secretion of IL-1β. The ATP-induced signaling cascade is regulated by nicotinic receptor stimulation. Phosphocholine and Phosphocholine-Modified Macromolecules modify many proteins and carbohydrates and reduce IL-1β. https://www.semanticscholar.org/papePhosphocholine-Modified-Macromolecules-and-Agonists-Hecker-Küllmae36c016ccf998540e4061f31581a91eae6feb780
Bromocytisine (3-BrCy) and 3-iodocytisine (3-ICy) exhibited increased binding affinity (especially at alpha7 nicotinic receptors; Ki approximately 0.1 microM) and functional potency. In addition, 3BrCy and 3-ICy increases intracellular Ca2+ in PC12 cells and inward currents in Xenopus oocytes expressing human alpha3beta4 nicotinic receptor (EC50 approximately 2 microM).
To examine the functional interaction between the sigma binding sites and nicotinic acetylcholine receptors, we investigated the effects of various sigma receptor ligands on nicotine-evoked Ca2+ uptake in differentiated PC12 cells. This study showed that PC12 cells express sigma 1-like sites and the inhibitory effect of sigma receptor ligands on the nicotine-evoked Ca2+ uptake was not directly coupled with either the sigma 1 or sigma 2 sites. https://www.sciencedirect.com/science/article/abs/pii/001429999600115X
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2024.11.30 03:29 Silly-Remove5789 Just had my first two attacks and the first one made me think I was going to die.

I am so sorry for this book I just wrote. I appreciate anyone who takes the time to read and response.
I'm not freaking out, at least trying not to. I'm at a point in my life where I'm just feeling blessed to be alive because I've beat a lot of really horrible things and I've already got some chronic conditions I'm learning how to live and age gracefully with. I've made a lot of progress with my life, with my health, and with my stress, so this was a really big surprise to me given the fact that I see lifestyle, diet, and exercise can affect this and while mine is far from perfect, it's only been improving. None the less, it's only giving me more motivation.
My first attack TERRIFIED me. Honest to goodness I thought the text I was sending my mom, which was barely coherent, was the last time I was ever going to speak with her. I thought maybe I was having an aneurysm or something. And the irony of what I was doing in that moment it started is hilarious and sad. I was literally sitting on my couch, smiling to myself, having like a mental breakthrough with how I viewed my health struggles and whether or not a long life was possible for me and I was totally telling myself that "yeah, I can, I'm gonna beat this, or at least make the most, put all my extra money into anything my body needs, do everything it takes" etc etc and then my heart did one big thump and started beating harder and faster than it has ever done in my entire life by a wide, wide margin. And I mean fucking POUNDING against every corner of my chest, i could feel my jugular was bulging especially on my left side, like i had a big fat frog throat, I felt like the blood pressure was building up in my head, I started getting nauseous, I started getting a really bad headache like my head was in a vice, since I didn't know what was happening I called 911, while I was on the phone with them my vision started going but it never left, I just felt like I was teetering on the edge of passing out, I wasn't sure if I gave them the correct phone number for myself and I know my phone number, I was having a hard time relaying my heart rate but it was at least 160. I remember my breath being very very calm, but shaky, and I remember starting to get cold pretty quick.
They got here in record fucking time. The whole thing must have not been much longer than 5 minutes and before they could get anything on me to get any vitals it stopped.
I have had a lot of medical trauma in the last 2 years, like severe. An ER department gave me temporary high output heart failure in March of '23 and I had to be admitted for a few days(please don't ask me about suing for negligence i don't want to have this conversation with anyone). I'm also in recovery so often get wrongly profiled and for 10 months I had a severe issue that no one could figure out and it always came to being profiled until i was finally vindicated. So all I could do was just look at them and just apologize and swear I'm not crazy and start sobbing.
Since I was so freaked out they tried to insist I go get checked out and I said I wanted to because nothing like that had ever happened before but ultimately my trauma got the best of me and also I do have medical training and by that point I already planted the seed in the back of my mind that it acted like an arrhythmia with the start and stop of it, it was just so brutal and lasted over 5 minutes and I really thought I was gonna black out and never wake up again as it was happening.
Over the last few days as it's happened my heart has felt kind of...er... sore? I thought maybe since it was so severe and kicked my ass so hard that maybe that's warranted. It would be nice to know if that's in any way to be expected.
I had another episode tonight and it was so much more fucking normal. Just started pounding, but not that crazy painful pounding against my chest that I never knew a heart could beat so hard like that with nausea and headache and cognitive decline and vision loss, just pounding. I did a passive leg lift after straining for 15 seconds in a semi recumbent position and it worked beautifully.
I am sorry for the rant. I just kind of can't believe life is doing me so dirty like this.
I appreciate anyone who stuck around to read all of this. I guess my questions are:
How bad really was that first episode? Was it dangerous or just inconvenient? Do you get episodes that severe? Is it normal to be sore after a severe episode?
Did you have to stop smoking weed/getting high? I read some rare cases of thc induced arrhythmia. No Im not suggesting that is my case at all, but I was admittedly high for the first one(i get high evenings after 7pm, I've been a chronic smoker for 24 years, used to all day every day, now just 3-4 evenings a week) I'm wondering if that's why it was so horrible. I haven't been high since and the second one still happened 4 days later.
Are yall on preventative medication? I don't really have high BP. It gets a little elevated with anxiety(i just got off 16 years of benzos in August), but clonidine treats me fine with that.
Do they do anything for this? Do I expect to be put on medication? I don't want to ever have a bad episode in front of my son, and if they're gonna be frequent then I'm kind of concerned. So I don't really know the options here. I see a cardiologist on Wednesday and I'm sure I'll be given an event monitor. I had one in the past and we recorded sinus tach, but I had to stop wearing it because my kid was at the age where they were just grabbing everything and needed held a lot. Also I have trace mitral or tricuspid valve regurgitation but as I understand it that isn't anything to be worried about either?
Again, thank you so much for reading my novel. 🙏
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2024.11.30 03:29 Routine_Business7872 TYPE 054B. [1079x438]

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2024.11.30 03:29 givenlover Ritsuka sees Mafuyu in the audience at the syh debut

This is the newest screen cap released from the Given movie To the Sea. The only scene that this one comes close to resembling from chapter 50 is a panel that follows after Ritsuka smiles at Mafuyu when he first sees him in the audience. When Ritsuka first sees Mafuyu, he looks so surprised then turns his head, blushing and gives him a beautiful smile. I don’t think this is that panel since the movie has been very true to the manga panels. Wherever this fits into the syh debut, Ritsuka looks incredibly happy and is blushing.
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2024.11.30 03:29 suckmycactuss Just need one referral!

Can you accept my invite, and help me get my gift? Just download the SHEIN app, and search for my code there! 6adsqvy4
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2024.11.30 03:29 sophia_the_2nd AITBF for not wanting to apologize to my boyfriend’s son after I picked up my toothbrush when he was at his dad’s?

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2024.11.30 03:29 saustin32 DTW to MCO March 2025

I’m looking to book a round trip flight from DTW to MCO March 2-8, 2025. 2 passengers, seat selection (middle is ok, just want to be next to eachother), carry ons. I cannot find anything under $900 after all the tax etc. which I find absurd.
I know predictions say 1-3 months out is the best time to book, but I worry that the better flight times and cheaper options will be gone by then. Any advice on what I should? What’s worked for you in the past? Let me know 🙏!
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2024.11.30 03:29 xatfi Keskuskauppakamari: Suomessa työskentelevien puolisoiden työluvan tulorajat ja saatavuusharkinta poistettava

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2024.11.30 03:29 Coreonix75 [Streamer Daily/Let's Game It Out]

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2024.11.30 03:29 Frosttrollgaming To the other subreddit sucking dick for attention

Please go back to sucking on Dales dick in private rather than trying to suck on everyone’s dick here. Everyone else, don’t give these strange dalesexual bot accounts the attention they desperately want and don’t visit their strange little cock sucking page unless it’s to mass troll or something but even then they don’t fucking deserve it.
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2024.11.30 03:29 FS_E54_Iron_Hollow Quick question

Is it okay if I dump all my SoUs into my weapons or rings instead of my armor? I told a friend about it and he said that it was dumb to not upgrade armor to at least +3, but I've never really thought about it till he mentioned it.
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2024.11.30 03:29 jr17th King Von X Key Glock X Comethazine

Enjoy
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2024.11.30 03:29 sadistapathy Burt is gonna find this particularly harder because of the nonstop sensory overload

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2024.11.30 03:29 Significant_Mark_809 Perfect shape

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2024.11.30 03:29 Extra_Fall_255 i kinda miss him

i just wish he didnt give up on us nor change in the first place. i miss how we were.
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2024.11.30 03:29 Only-Survey-5319 STAR-BD5P-SWGD for 5,000 UEC

Just use the code or this direct link and enjoy the game :)
https://robertsspaceindustries.com/enlist?referral=STAR-BD5P-SWGD
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2024.11.30 03:29 ITAKEJOKESSEROUSLY DBMRonpa will not feature any animation

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2024.11.30 03:29 axchieve I AM WORRIED, SO HELP !!!!!!!!

my battery percentage is dropping so fast, what can i do ?? it drops 3% in 2 months, is this normal or not l I am worried….
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2024.11.30 03:29 Ok_Muffin3446 Audi Q5 CarPlay

Just wondering if anyone knows of a good retrofit unit for a 1st gen Audi Q5 that has concert system. Would prefer a retrofit for a cleaner look
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2024.11.30 03:29 BackwoodsSensei How do I invite my friends back to my session?

Doing a coop 2v2 game with a friend and he lagged out. Now I can’t out how to invite him back! Pls help
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2024.11.30 03:29 Specialist-Banana-51 You have to be kidding me 😭

Someone please liberate me from this hell
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https://google.com/